Invitrogen™ Mu-Calpain Monoclonal Antibody (9A4H8D3)

Catalog No.	Quantity
MA3940	100 μg

Catalog No. MA3940 Supplier Invitrogen™ Supplier No. MA3940

Description

Mouse Monoclonal Antibody

MA3-940 detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, mouse lung, pig cultured cells and hamster and rabbit samples. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin. MA3-940 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunocytochemistry procedures. By Western blot, this antibody detects an 80 kDa protein representing mu-calpain from human platelets and erythrocytes and HeLa and A431 cell lysates. Immunocytochemical staining of mu-calpain in LLC-PK1 cells with MA3-940 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments. The MA3-940 antigen is purified bovine skeletal muscle 80 kDa mu-calpain subunit. This antibody recognizes an epitope between amino acids 465-520 (domain III) of human mu-calpain.

The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.

Specifications

• Antigen: Mu-Calpain
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 9A4H8D3
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene: CAPN1
• Gene Accession No.: O35350, P06815, P07384, P35750, P97571, Q27970
• Gene Alias: calcium activated neutral protease; calcium-activated neutral protease 1; calcium-activated neutral proteinase 1; calcium-dependent protease; calpain; calpain 1; calpain 1 large subunit; calpain 1, (mu/I) large subunit; calpain 1, large subunit; calpain mu-type; calpain, large polypeptide L1; calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain-I; Calpain-II; CANP; CANP 1; Canp1; CANPL1; Capa1; Capa-1; Capn1; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; Cls1; I79_024883; micromolar calcium-activated neutral protease 1; micromolar calcium-activated neutral protease 1 isoform A; micromolar calcium-activated neutral protease 1 isoform B; micromolar calcium-dependent neutral protease large subunit; micromolar-calpain; mucalpain; mu-calpain; mu-calpin; muCANP; muCL; PIG30; SPG76
• Gene Symbols: CAPN1
• Host Species: Mouse
• Immunogen: Purified bovine skeletal muscle 80 kDa mu-calpain subunit.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100009089, 100758636, 12333, 281661, 29153, 397027, 823
• Target Species: Bovine, Hamster, Human, Mouse, Pig, Rabbit, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1