Invitrogen™ Musashi-2 Monoclonal Antibody (C1), eFluor™ 660, eBioscience™

Catalog No.	Quantity
50-967-782	100 μg

Catalog No. 50-967-782 Supplier Invitrogen™ Supplier No. 50967782

Description

Mouse Monoclonal Antibody

The monoclonal antibody C1 recognizes human and mouse Musashi-2 (Msi2), a highly conserved RNA-binding protein. Musashi-2 contains two RNA-recognition domains and is expressed as one of two isoforms of 36 and 37kDa. Musashi 2 with its family member Musashi-1, are expressed at high levels in stem and tumor cells. During neural development, Musashi-2 is expressed in progenitor cells in the ventricular and subventricular zones, while postnatally, Musashi-2 expression is limited to multiple subsets of neurons and astrocytes in addition to adult stem cell populations. Functionally, Musashi-2 associates with Sox2 and is required for self-renewal of embryonic stem cells. Much like its role in maintaining stemness in neural progenitors, Musashi-2 is expressed in hematopoetic stem cells and plays a similar role in maintaining self-renewal. Musashi-2 has been found to be overexpressed in human myeloid leukemia and the levels of expression correlate with decreased survival. Musashi-2 may serve as a prognostic marker for acute myeloid leukemia (AML). Applications Reported: This C1 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This C1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody...

Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to posttranslational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events.Tissue specificity: Ubiquitous; detected at low levels.

Specifications

• Antigen: Musashi-2
• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Monoclonal
• Clone: C1
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 660
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: MSI2
• Gene Accession No.: Q920Q6, Q96DH6
• Gene Alias: 1700105C15Rik; AI451722; AI563628; AW489193; MSI2; Msi2h; musashi homolog 2; musashi RNA binding protein 2; musashi RNA-binding protein 2; Musashi2; musashi-2; RGD1560397; RNA-binding protein Musashi homolog 2
• Gene Symbols: MSI2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 124540, 76626
• Target Species: Human, Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG3 κ

Frequently Asked Questions (FAQs)

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

Is the eFluor 660 fluorochrome compatible with Anti-Cy5/Alexa Fluor 647 beads?

Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.