Invitrogen™ MUTYH Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA527855

Catalog No. PIPA527855

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

MYH (mutY homolog (E. coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.

Specifications

• Antigen: MUTYH
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MUTYH
• Gene Alias: 5730495A01Rik; A/G-specific adenine DNA glycosylase; adenine DNA glycosylase; adenine-DNA glycosylase; hMYH; MGC4416; mMYH; mutY DNA glycosylase; mutY homolog; mutY homolog (E. coli); mutY homolog alpha; Mutyh; Mutyha; Mutyhalpha; Mutyhb; Mutyhbeta; Mutyhc; mutY-like protein; Myh; OTTHUMP00000009098; OTTHUMP00000009102; rMYH; RP4-534D1.2; the full-length form type A, 5' region
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4595, 70603
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 0.16 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q99P21, Q9UIF7
• Gene Symbols: MUTYH
• Immunogen: Recombinant fragment corresponding to a region within amino acids 257 and 546 of Human MUTYH.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG