Invitrogen™ Myeloperoxidase (MPO) Monoclonal Antibody (MPO455-8E6), PE, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 12129942

Catalog No. 50-245-285

Description

Description: The monoclonal antibody MPO455-8E6 recognizes myeloperoxidase (MPO), a protein within the azurophilic granules of myeloid cells. MPO is a multimeric protein comprised of two 55 kDa subunits and two 15 kDa subunits. The larger subunits associate with a heme protein resulting in a greenish color. As an enzyme, MPO breaks down hydrogen peroxide and oxidizes tyrosine. The products of this reaction, hypochlorous acid and tyrosyl radical, causethe cytotoxic and killing effects characteristic of neutrophils. Myeloperoxidase is important in the diagnosis of some cancers and increases in serum levels have been shown to correlate with cardiac events. Applications Reported: This MPO455-8E6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MPO455-8E6 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeablization Buffer Set (Product # 88-8824-00). For best results, whole blood should first be stained with antibodies to surface antigens then treated with 1X RBC lysis buffer (Product # 00-4333-57) to lyse erythrocytes. Then, for intracellular staining for MPO, cells should be fixed with IC Fixation Buffer (Product # 00-8222-49) washed two times with Permeabilization Buffer (Product # 00-8333-56) and then incubated with MPO455-8E6 for 30-60 minutes. After washing, cell...

Myeloperoxidase (MPO) is a heme protein that is synthesized during myeloid differentiation and is a major component of neutrophil azurophilic granules. It is produced as a single chain precursor and subsequently cleaved into a light and heavy chain. The mature MPO is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme is responsible for producing hypohalous acids that are central to the microbicidal activity of neutrophils. Diseases that are associated with MPO include Myeloperoxidase Deficiency and Alzheimer's Disease, Familial, 1.

Specifications

• Antigen: Myeloperoxidase (MPO)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05164
• Gene Symbols: MPO
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4353
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid
• Green Features: Responsibly packaged

• Applications: Flow Cytometry
• Clone: MPO455-8E6
• Conjugate: PE
• Gene: MPO
• Gene Alias: 84 kDa myeloperoxidase; 89 kDa myeloperoxidase; mKIAA4033; MPO; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1