Myeloperoxidase Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 2004181

Catalog No. 50-199-1053

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes]. Cross reactivity against Myeloperoxidase from other tissues and species may occur but have not been specifically determined.

Myeloperoxidase (MPO) is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains with a molecular weight range of 59 - 64 kDa, and two unglycosylated beta chains (MW ∼14 kDa) with total MW ∼150 kDa and a theoretical pI of 9.2. Traditionally, myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities such as periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently, it was shown that myeloperoxidase participates in the initiation and progression of cardiovascular disease. Myeloperoxidase possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers. Myeloperoxidase is part of the host defense system of polymorphonuclear leukocytes and myeloperoxidase is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN (polymorphonuclear leukocytes), MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Specifications

• Antigen: Myeloperoxidase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MPO
• Gene Alias: 84 kDa myeloperoxidase; 89 kDa myeloperoxidase; mKIAA4033; MPO; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain
• Host Species: Rabbit
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 5 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% gentamicin sulfate; pH 7.2
• Gene Accession No.: P05164
• Gene Symbols: MPO
• Immunogen: Myeloperoxidase [Human Leukocytes]
• Regulatory Status: RUO
• Gene ID (Entrez): 4353
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized