MyoD Antibody (MYD712), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP232883A	Novus Biologicals NBP2328830.1MG	0.1 mg
NBP232883B	Novus Biologicals NBP2328830.2MG	0.2 mg

Description

MyoD Monoclonal specifically detects MyoD in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Single Cell Western.

Specifications

• Antigen: MyoD
• Dilution: Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P15172
• Gene ID (Entrez): 4654
• Immunogen: Recombinant human MyoD1 protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 45 kDa

• Clone: MYD712
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cell Cycle and Replication, Growth and Development, Phospho Specific
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
• Gene Symbols: MYOD1
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.