Myogenin Mouse, Clone: F5D, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP232986F	Novus Biologicals NBP2329860.02MG	0.02 mg
NBP232986A	Novus Biologicals NBP2329860.1MG	0.1 mg
NBP232986B	Novus Biologicals NBP2329860.2MG	0.2 mg

Description

Myogenin Monoclonal specifically detects Myogenin in Human, Mouse, Rat, Porcine, Canine, Feline samples. It is validated for Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Myogenin
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Stem Cell Markers, Transcription Factors and Regulators
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: BHLHC3, bHLHc3Myogenic factor-4; myogenin, Class C basic helix-loop-helix protein 3, myf-4, MYF4myogenin, Myogenic factor 4, MYOGENIN, myogenin (myogenic factor 4)
• Gene Symbols: MYOG
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

• Clone: F5D
• Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Feline
• Gene Accession No.: P15173
• Gene ID (Entrez): 4656
• Immunogen: Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 34 kDa