MYPT1 Mouse anti-Human, Clone: 2A1A9, Proteintech

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-173-6928	Proteintech Group Inc 665061IG150UL	150 μL

Description

MYPT1, Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: MYPT1
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 50% glycerol and 0.1% sodium azide; pH 7.3
• Gene Alias: MBS, MYPT1, PPP1R12A
• Gene Symbols: PPP1R12A
• Isotype: IgG3
• Purification Method: Protein A
• Product Type: Antibody

• Clone: 2A1A9
• Applications: Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: O14974
• Gene ID (Entrez): 4659
• Immunogen: MYPT1 Fusion Protein Ag17496
• Primary or Secondary: Primary
• Content And Storage: -20°C
• Gene: PPP1R12A