N-Me-6,7-diOH-TIQ Antibody, R&D Systems™

Catalog No.	Quantity
MAB167SP	25 μg

Catalog No. MAB167SP Supplier R&D Systems Supplier No. MAB167SP

Description

Mouse Monoclonal Antibody

N-Me-6,7-diOH-TIQ Monoclonal specifically detects N-Me-6,7-diOH-TIQ in Multi-Species samples. It is validated for Immunohistochemistry.

Specifications

• Antigen: N-Me-6,7-diOH-TIQ
• Applications: Immunohistochemistry
• Classification: Monoclonal
• Clone: 106814
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry 8-25 ug/mL
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Host Species: Mouse
• Immunogen: Carrier protein-coupled N-Me-6,7-diOH-TIQ
• Purification Method: Protein A or G purified from ascites
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes N-Me-6,7-diOH-TIQ in immunoassays. The specificity was determined by a competition type assay where the N-Me-6,7-diOH-TIQ labeled with biotin at position 5 or 8 competed with another compound for binding to the antibody binding site. The detection limit of biotinylated N-Me-6,7-diOH-TIQ in assays where the molecule was captured by the antibody coated on microplates and developed by HRP-streptavidin was 5 nM. The dissociation constants of the interaction between the antibody and N-Me-6,7-diOH-TIQ and its biotinylated analog were 0.2 and 0.5 μM, respectively. This antibody specifically recognizes N-Me-6,7-diOH-TIQ and shows 60% cross-reactivity with 6,7-diOH-TIQ, 15% cross-reactivity with 1,N-di-Me-6,7-diOH-TIQ, 3% cross-reactivity with 1-Me-6,7-diOH-TIQ, and less than 1% or no cross-reactivity with tetrahydroisoquinoline (TIQ), 5-OH-isoquiniline, 5-OH-N-Me-isoquinolinium, tetrahydropapaveroline, 9H-pyrido[3,4-b]indole, and 1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole. The percent cross-reactivity is expressed by the relative value, which produced 50% inhibition of interaction between the biotin-labeled N-methyl-6,7-dihydroxytetrahydroisoquinoline and the other compound.
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Target Species: Multi-species
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Form: Purified
• Isotype: IgG2b

For Research Use Only