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Invitrogen™ NA-Star™ Influenza Neuraminidase Inhibitor Resistance Detection Kit
Description
Includes
Substrate, required buffer and reaction accelerator
Rapid and sensitive quantitation of influenza neuraminidase inhibitor resistance in dilutions of virus culture medium in a 96-well microplate format. The NA-Star Influenza Neuraminidase Inhibitor Resistance Detection Kit includes everything needed to qua
The NA-Star™ Influenza Neuraminidase Inhibitor Resistance Detection Kit is designed for the rapid and sensitive quantitation of influenza neuraminidase inhibitor resistance in dilutions of virus culture medium in a 96-well microplate format. The NA-Star™ Influenza Neuraminidase Inhibitor Reagent kit provides all necessary assay reagents enabling improved global assay standardization and more accurate comparison of results lab-to-lab.
The NA-Star™ Influenza Neuraminidase Inhibitor Resistance Detection Kit includes everything you need to quantitate neuraminidase activity and neuraminidase inhibitor resistance in avian, equine, human (types A and B), and porcine influenza viruses. The kit's fast and easy protocol and convenient 96-well plate format make it ideal for monitoring influenza virus neuraminidase inhibitor resistance, as well as high-throughput inhibitor compound screening.
- Includes pre-made solutions for all required reagents, enabling you to standardize assays and compare results between labs easily.
- Provides up to 50-fold higher sensitivity than assays using fluorescent methylumbelliferone N-acetylneuraminic acid (MUNANA) substrate.
- Dynamic range of four orders of magnitude enables you to quantitate virus isolates over a broad range of virus concentration and with varying levels of neuraminidase activity without performing numerous sample dilutions.
- Fast and easy protocol enables you to complete assays in less than 1.5 hours, providing rapid assay throughput.
Complete Kit for Measuring the Neuraminidase Inhibitor Resistance of Influenza Viruses
The NA-Star™ Influenza Neuraminidase Inhibitor Resistance Detection Kit includes NA-Star chemiluminescent substrate for neuraminidase, all necessary assay reagents, and microplates -- everything you need for the fast, accurate quantitation of neuraminidase inhibitor resistance in influenza virus isolates.
Get Results in Less than 1.5 Hours
The kit's fast and easy protocol enables you to perform assays in less than 1.5 hours. Simply incubate your virus samples with dilutions of neuraminidase inhibitor, add NA-Star chemiluminescent substrate, incubate again, and then add the accelerator solution, which triggers light emission from the reaction product. Light signal is measured with a luminometer, including multi-mode instruments that include a luminometer mode. For best results, use a luminometer with an automatic injector to add the accelerator solution. You may use a luminometer without an injector, provided you add reagents with a multichannel pipettor and read the plate immediately.
Up to 50-Fold Higher Sensitivity than MUNANA-Based Assays
The kit's chemiluminescent-based detection technology provides a wide dynamic range -- greater than four orders of magnitude of neuraminidase concentration (two orders of magnitude greater than fluorescent MUNANA-based assays) -- enabling you to quantitate neuraminidase inhibitor resistance levels over a broad range of virus concentration and neuraminidase activity without having to test multiple virus dilutions.
Pre-Made Reagents Make it Easy to Standardize Assays
Quality-tested protocols, microplates, and pre-made reagents eliminate variability, enabling you to easily standardize assay performance and results across experiments and different laboratories.
Monitor Drug Resistance of Human, Avian, and Livestock Influenza Viruses
The kit's ease of use, standardization, and broad range of compatible species (avian, equine, human types A and B, and porcine influenza viruses) make it an important new tool for assessing and researching drug resistance of influenza in humans, birds, and livestock.
Screen for New Inhibitors and Develop New Vaccines
The kit has many other applications, including screening for new neuraminidase inhibitors and quantitating viral neuraminidase activity for vaccine development. It is also ideal for quantitating neuraminidases from bacteria, including S. typhimurium, C. perfringens, V. cholera, and likely others.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Specifications
| Content And Storage | This kit contains the enzyme substrate, assay buffer, reaction accelerator, and microplates. Store reagent set components at 2°C to 8°C. |
| Detection Method | Luminescence |
| For Use With (Application) | Microbiological Analysis |
| For Use With (Equipment) | Luminometer |
| Product Type | Neuraminidase Assay |
| Form | Liquid |
| Format | 96-well plate |
| Product Line | NA-Star, NovaBright |
| Quantity | 1 kit |
| Substrate | NA-Star™ |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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