Invitrogen™ NALCN Monoclonal Antibody (N187/7), PE

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545575

Catalog No. PIMA545575

Description

1 μg/mL of MA5-45575 was sufficient for detection of NALCN in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 200kDa. This antibody was formerly sold as clone S187-7.

Nalcn is a voltage-independent, cation-nonselective channel which is permeable to sodium, potassium and calcium ions. Nalcn regulates the resting membrane potential and controls neuronal excitability (PubMed:17448995). Neuropeptides such as neurotensin and substance P (SP) stimulate the firing of action potentials by activating NALCN through a SRC family kinases-dependent pathway. In addition to its baseline activity, Nalcn activity is enhanced/modulated by several GPCRs, is required for normal respiratory rhythm and neonatal survival, is involved in systemic osmoregulation by controlling the serum sodium concentration. Nalcn is partly responsible for the substance P-induced depolarization and regulation of the intestinal pace-making activity in the interstitial cells of Cajal. Further, Nalcn plays a critical role in both maintenance of spontaneous firing of substantia nigra pars reticulata (SNr) neurons and physiological modulation of SNr neuron excitability. Diseases associated with NALCN include Congenital contractures of the limbs and face, Hypotonia, Developmental Delay and Hypotonia, Infantile Psychomotor Retardation And Characteristic Facies 1.

Specifications

• Antigen: NALCN
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48 mM MES, 95.64 mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: Q6Q760, Q8BXR5, Q8IZF0
• Gene Symbols: NALCN
• Immunogen: Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot, Immunocytochemistry
• Clone: N187/7
• Conjugate: PE
• Gene: NALCN
• Gene Alias: A530023G15Rik; AI849508; bA430M15.1; brain voltage-gated cation channel; CanIon; CLIFAHDD; FLJ23913; FLJ44659; FLJ44764; Four domain-type voltage-gated ion channel alpha-1 subunit; four repeat voltage-gated ion channel; IHPRF; IHPRF1; INNFD; MGC74524; Nalcn; Nca; Rb21; Rb21-channel; Sodium leak channel non-selective protein; sodium leak channel, non selective; sodium leak channel, non-selective; Vgcnl1; voltage gated channel like 1; voltage gated channel-like protein 1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 259232, 266760, 338370
• Content And Storage: 4°C
• Form: Liquid