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Invitrogen™ NAMPT Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P222BT25UG

Encompass_Preferred

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Catalog No. 500P222BT25


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Description

Description

AA Sequence of recombinant protein: MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Visfatin. Anti-Human Visfatin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human Visfatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Visfatin (500-P222) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Visfatin. Western Blot: To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Visfatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P222BT-1MG will be provided as 2 x 500 μg

Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

NAMPT
Polyclonal
Biotin
NAMPT
1110035O14Rik; AI314458; AI480535; DKFZp666B131; EC 2.4.2.12; hypothetical protein; MGC117256; NAmPRTase; NAMPT; nampt1; nampta; NAMPTP1; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase 1; nicotinamide phosphoribosyltransferase a; nicotinamide phosphoribosyltransferase pseudogene 1; PBEF; Pbef1; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; pre-B-cell colony-enhancing factor 1 precursor; VF; VISFATIN
Rabbit
Antigen affinity chromatography
RUO
10135
-20°C
Lyophilized
ELISA, Western Blot
0.1-1.0 mg/mL
PBS with no preservative
P43490
NAMPT
E.coli-derived, 52.6 kDa Recombinant Human Visfatin.
25 μg
Primary
Human
Antibody
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