NAMPT Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P22250UG	Invitrogen™ 500P22250UG	50 μg
500P222100	Invitrogen™ 500P222100UG	100 μg
500P2221MG	Invitrogen™ 500P2221MG	1 mg

Description

AA Sequence of recombinant protein: MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Visfatin. Anti-Human Visfatin-specific antibody was purified by affinity chromatography employing an immobilized Human Visfatin matrix. Sandwich ELISA: To detect Human Visfatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Visfatin (500-P222Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Visfatin. Western Blot: To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Visfatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.

Specifications

• Antigen: NAMPT
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P43490
• Gene ID (Entrez): 10135
• Immunogen: E.coli-derived Recombinant Human Visfatin.
• Purification Method: Antigen affinity chromatography
• Gene: NAMPT

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: 1110035O14Rik; AI314458; AI480535; DKFZp666B131; EC 2.4.2.12; hypothetical protein; MGC117256; NAmPRTase; NAMPT; nampt1; nampta; NAMPTP1; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase 1; nicotinamide phosphoribosyltransferase a; nicotinamide phosphoribosyltransferase pseudogene 1; PBEF; Pbef1; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; pre-B-cell colony-enhancing factor 1 precursor; VF; VISFATIN
• Gene Symbols: NAMPT
• Primary or Secondary: Primary
• Content And Storage: -20°C