Invitrogen™ NANOS3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA520537

Catalog No. PIPA520537

Description

A suggested positive control is human brain tissue lysate. PA5-20537 can be used with blocking peptide PEP-0657.

Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.

Specifications

• Antigen: NANOS3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NANOS3
• Gene Alias: nanos C2HC-type zinc finger 3; nanos homolog 3; nanos homolog 3 (Drosophila); NANOS1L; NANOS3; NOS3; NOS-3; ZC2HC12C
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 244551, 288909, 342977
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: P60323, P60324
• Gene Symbols: NANOS3
• Immunogen: A 16 amino acid peptide near the carboxy terminus of the human Nanos3.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov