Invitrogen™ NDUFS5 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143914

Catalog No. PIPA5143914

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human K562 whole cell, human HepG2 whole cell, human RT4 whole cell, human Hela whole cell, rat liver tissue, rat heart tissue, mouse liver tissue, mouse heart tissue. IHC: human liver cancer tissue, human thyroid cancer tissue, human colorectal adenocarcinoma tissue, mouse heart tissue, rat heart tissue. Flow: Hela cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Located in the mitochondrial inner membrane, mitochondrial complex I is the first and largest enzyme in the electron transport chain of oxidative phosphorylation. By oxidizing NADH that is produced in the Krebs cycle, this complex utilizes the two electrons to reduce ubiquinone to ubiquinol, thereby initiating the passage of electrons to successive complexes and ultimately leading to the reduction of oxygen to water. Mitochondrial complex I consists of over 40 subunits and is of considerable clinical interest since defects in any of the subunits can lead to various myopathies and neuropathies. As a subunit of mitochondrial complex I, NDUFS5 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5), also designated NADH-ubiquinone oxidoreductase 15 kDa subunit, is a 106 amino acid protein that is suggested to not be involved in catalysis. NDUFS5 is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart.

Specifications

• Antigen: NDUFS5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NDUFS5
• Gene Alias: AI256693; CI-15 kDa; CI15K; CI-15k; Complex I-15 kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 5; NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase); NADH dehydrogenase (ubiquinone) Fe-S protein 5b, 15kDa (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 5; NADH:ubiquinone oxidoreductase 15 kDa IP subunit; NADH:ubiquinone oxidoreductase subunit S5; NADH-ubiquinone oxidoreductase 15 kDa subunit; Ndufs5; Ndufs5b
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 362588, 4725, 595136
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: O43920, Q99LY9
• Gene Symbols: NDUFS5
• Immunogen: E.coli-derived human NDUFS5 recombinant protein (Position: M1-P106).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG