Invitrogen™ Nectin 3 Monoclonal Antibody (103-A1)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA533378

Catalog No. PIMA533378

Description

Product is supplied at 1 mL (100 μL/mL). Positive control: L-cells (A cultured line of C3H mouse fibroblasts); Negative control: Cells/tissues derived from nectin-3 knockout mouse.

Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calciumindependent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3 alpha, -3 beta and -3 gamma, of which nectin-3 alpha is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.

Specifications

• Antigen: Nectin 3
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 0.1% BSA and no preservative
• Gene Accession No.: Q9JLB9
• Gene Symbols: NECTIN3
• Immunogen: Recombinant mouse nectin-3 extracellular domain (50-400 aa) fused to IgG Fc.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a

• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Clone: 103-A1
• Conjugate: Unconjugated
• Gene: NECTIN3
• Gene Alias: 2610301B19Rik; 3000002N23Rik; 4921513D19Rik; AA407785; AU016832; AW538082; CD113; CDW113; DKFZP566B0846; FLJ90624; nectin 3; nectin cell adhesion molecule 3; Nectin3; Nectin-3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; PPR3; PRR3; PVRL3; PVRR3
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 58998
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

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