Invitrogen™ Nectin 3 Monoclonal Antibody (103-A1), FITC

Catalog No.	Quantity
FERMA549077	100 μg

Catalog No. FERMA549077 Supplier Invitrogen™ Supplier No. MA549077

Description

Rat Monoclonal Antibody

Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actinfilaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation ofmaturing spermatids form the seminiferous epithelium.

Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calciumindependent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3 alpha, -3 beta and -3 gamma, of which nectin-3 alpha is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.

Specifications

• Antigen: Nectin 3
• Applications: Flow Cytometry, Functional Assay, Inhibition Assays, Immunohistochemistry (Frozen), Immunocytochemistry
• Classification: Monoclonal
• Clone: 103-A1
• Concentration: 100 μg/mL
• Conjugate: FITC
• Formulation: PBS with 1% BSA and 0.02% sodium azide
• Gene: NECTIN3
• Gene Accession No.: Q9JLB9
• Gene Alias: 2610301B19Rik; 3000002N23Rik; 4921513D19Rik; AA407785; AU016832; AW538082; CD113; CDW113; DKFZP566B0846; FLJ90624; nectin 3; nectin cell adhesion molecule 3; Nectin3; Nectin-3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; PPR3; PRR3; PVRL3; PVRR3
• Gene Symbols: NECTIN3
• Host Species: Rat
• Immunogen: The monoclonal antibody 103-A1 recognizes mouse nectin-3.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 58998
• Target Species: Mouse
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a