Invitrogen™ Nectin 3 Monoclonal Antibody (02)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA529440

Catalog No. PIMA529440

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human Nectin 3.

Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calciumindependent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3 alpha, -3 beta and -3 gamma, of which nectin-3 alpha is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.

Specifications

• Antigen: Nectin 3
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q9NQS3
• Gene Symbols: NECTIN3
• Immunogen: Recombinant Human CD113/Nectin-3 protein (Met1-Asp400).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin)
• Clone: 02
• Conjugate: Unconjugated
• Gene: NECTIN3
• Gene Alias: 2610301B19Rik; 3000002N23Rik; 4921513D19Rik; AA407785; AU016832; AW538082; CD113; CDW113; DKFZP566B0846; FLJ90624; nectin 3; nectin cell adhesion molecule 3; Nectin3; Nectin-3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; PPR3; PRR3; PVRL3; PVRR3
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 25945
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid