Invitrogen™ Nectin 3 Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA547441

Catalog No. PIPA547441

Description

In direct ELISAs, less than 65% cross-reactivity with recombinant mouse Nectin-3 is observed and less than 5% cross-reactivity with recombinant human (rh) Nectin-1, rhNectin-2, and rhNectin-4 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calciumindependent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3 alpha, -3 beta and -3 gamma, of which nectin-3 alpha is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.

Specifications

• Antigen: Nectin 3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NECTIN3
• Gene Alias: 2610301B19Rik; 3000002N23Rik; 4921513D19Rik; AA407785; AU016832; AW538082; CD113; CDW113; DKFZP566B0846; FLJ90624; nectin 3; nectin cell adhesion molecule 3; Nectin3; Nectin-3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; PPR3; PRR3; PVRL3; PVRR3
• Host Species: Goat
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 25945
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q9NQS3
• Gene Symbols: NECTIN3
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Nectin-3 Leu56-Asp400.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG