Invitrogen™ NEDD8 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA517476

Catalog No. PIPA517476

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other ubiquitin family members, including ubiquitin, SUMO1, SUMO2, SUMO3 and ISG15.

NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Specifications

• Antigen: NEDD8
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Nedd8
• Gene Alias: nedd8; NEDD-8; NEDD8 ubiquitin like modifier; nedd8.L; nedd8.S; neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; neural precursor cell expressed, developmentally down-regulated 8 L homeolog; neural precursor cell expressed, developmentally down-regulated 8 S homeolog; neural precursor cell expressed, developmentally down-regulated gene 8; neural precursor cell-expressed developmentally down-regulated; Rub1; si:zc14a17.5; ubiquitin; ubiquitin-like protein Nedd8; XELAEV_18007009mg
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18002, 25490, 4738
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 162 μg/mL
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene Accession No.: P29595, Q15843, Q71UE8
• Gene Symbols: Nedd8
• Immunogen: Synthetic peptide corresponding to amino acids at the amino-terminus of human NEDD8 protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG