Invitrogen™ NEFM Polyclonal Antibody

Catalog No.	Quantity
APPA6758	250 μL

Catalog No. APPA6758 Supplier Invitrogen™ Supplier No. PA116758

Description

Chicken Polyclonal Antibody

Suggested positive control: antigen standard for NEFM (transient overexpression lysate).

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Specifications

• Antigen: NEFM
• Applications: Immunohistochemistry (Free Floating), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: Tissue culture supernatant with 0.035% sodium azide
• Gene: NEFM
• Gene Accession No.: O77788, P07197, P08552, P08553, P12839, P16053
• Gene Alias: 160 kDa neurofilament protein; major neuronal intermediate filament subunit; medium neurofilament subunit; middle molecular weight neurofilament protein NF-M(2); Nef3; Nefm; nef-m; nefm.S; nefm-a; nefm-b; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium; neurofilament medium polypeptide; neurofilament medium S homeolog; neurofilament medium tail domain; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neurofilament-M; neurofilament-M subunit; neuronal intermediate filament; NF160; NF165; NFM; NF-M; NF-M c-terminus; NF-M protein; NMC; XELAEV_18020229mg
• Gene Symbols: NEFM
• Host Species: Chicken
• Immunogen: Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.
• Purification Method: IgY fraction
• Quantity: 250 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100054129, 100625407, 18040, 24588, 281347, 396206, 4741
• Target Species: Human, Mouse, Rat, Bovine, Pig, Horse, Chicken, Drosophila
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgY