Invitrogen™ Nephrin Monoclonal Antibody (C14), Invitrogen™

Catalog No.	Quantity
FEMA550998	20 μL
FEMA550997	200 μL

Catalog No. FEMA550998 Supplier Invitrogen™ Supplier No. MA550998

Description

Mouse Monoclonal Antibody

Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling. Nephrin seems to play a role in the development or function of the kidney glomerular filtration barrier and it may anchor the podocyte slit diaphragm to the actin cytoskeleton.

Specifications

• Antigen: Nephrin
• Applications: Western Blot, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: C14
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.05% Proclin 300; pH 7.4
• Gene: Nphs1
• Gene Accession No.: O60500, Q9QZS7, Q9R044
• Gene Alias: CNF; Nephrin; nephrin 1; NephrinB; nephrosis 1 homolog, nephrin; nephrosis 1, congenital, Finnish type (nephrin); nephrosis 1, nephrin; NPHN; NPHS1; NPHS1 nephrin; NPHS1, nephrin; renal glomerulus-specific cell adhesion receptor
• Gene Symbols: Nphs1
• Host Species: Mouse
• Immunogen: Recombinant Human NPHN protein, Arg1160-Val1241 (Accession #O60500).
• Purification Method: Protein A/G
• Quantity: 20 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4868, 54631, 64563
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ