Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-168-30	Invitrogen™ 53984380	25 μg
50-168-31	Invitrogen™ 53984382	100 μg

Description

Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody. Applications Reported: This 10C2 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining, and flow cytometric analysis. Applications Tested: This 10C2 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

Specifications

• Antigen: Nestin
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
• Gene Symbols: Nes
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Nes

• Clone: 10C2
• Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry
• Conjugate: Alexa Fluor 488
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P48681
• Gene ID (Entrez): 10763
• Isotype: IgG1
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.