Invitrogen™ Nestin Monoclonal Antibody (10C2), eFluor™ 570, eBioscience™

Catalog No.	Quantity
41-984-380	25 μg

Catalog No. 41-984-380 Supplier Invitrogen™ Supplier No. 41984380

Description

Mouse Monoclonal Antibody

The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody. Applications Reported: This 10C2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This 10C2 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized human cells and can be used at less than or equal to 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission:...

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

Specifications

• Antigen: Nestin
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Monoclonal
• Clone: 10C2
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 570
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Nes
• Gene Accession No.: P48681
• Gene Alias: AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
• Gene Symbols: Nes
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10763
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Frequently Asked Questions (FAQs)

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.