Invitrogen™ Nestin Monoclonal Antibody (2Q178)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA191657

Catalog No. PIMA191657

Description

For use in ICC applications, cells can be fixed with 4% paraformaldehyde buffered with 50mM sodium borate at p.H. 9.5. for IHC-P and IHC-Fr tissue can be fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. The predicted molecular weight of MA1-91657 is ~200kDa.

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

Specifications

• Antigen: Nestin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 0.05% sodium azide
• Gene Accession No.: P21263, Q6P5H2
• Gene Symbols: Nes
• Immunogen: Full length native Nestin protein purified from embryonic rat spinal cord.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 2Q178
• Conjugate: Unconjugated
• Gene: Nes
• Gene Alias: AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 18008, 25491
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid