Invitrogen™ Nestin Monoclonal Antibody (3k1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA126777

Catalog No. PIMA126777

Description

Recommended positive controls: Human neural progenitors and A172 cells. MA1-26777 will not react with rodent. Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot- add 50% glycerol and store at -20ºC or below. Avoid freeze-thaw cycles.

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

Specifications

• Antigen: Nestin
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and no preservative; pH 7.4
• Gene Accession No.: P48681
• Gene Symbols: Nes
• Immunogen: NS0 cells transfected with a fragment corresponding to residues 618-1618 of human Nestin.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: 3k1
• Conjugate: Unconjugated
• Gene: Nes
• Gene Alias: AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 10763
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid