Invitrogen™ Nestin Monoclonal Antibody (Rat-401 (4D4))

Catalog No.	Quantity
MA181819	100 μg

Catalog No. MA181819 Supplier Invitrogen™ Supplier No. MA181819

Description

Mouse Monoclonal Antibody

This antibody does not react with human. In Western blot, this antibody detects a ~200-220 kDa band in newborn rat or mouse cell extracts. For immunohistochemistry, we recommend perfusing tissues with 4% paraformaldehyde (pH 7.4) for light microscopy and with either 4% paraformaldehyde (pH 10.0) or 4% paraformaldehyde with 0.1% glutaraldehyde (pH 7.4) for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50mM sodium borate (pH 9.5). For Western blot, it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested. Mouse anti Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985).

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

Specifications

• Antigen: Nestin
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: Rat-401 (4D4)
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.05% sodium azide
• Gene: Nes
• Gene Accession No.: P21263, Q6P5H2
• Gene Alias: AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
• Gene Symbols: Nes
• Host Species: Mouse
• Immunogen: Nestin purified from embryonic rat spinal cord.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18008, 25491
• Target Species: Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1