Invitrogen™ Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 14584382

Catalog No. 50-132-06

Description

Description: The monoclonal antibody Rat-401 recognizes mouse and rat nestin, a Class VI intermediate filament protein (>170 kDa) primarily expressed in neuroepithelial stem cells. Upon neural differentiation, expression is down-regulated and within the adult nervous system is limited to progenitor cells in the cortical subventricular zone, the hippocampal dentate gyrus, dorsal root ganglia satellite cells, and a subpopulation of Schwann cells. Nestin expression is also present in other progenitor cell populations such as pancreatic islet, angiogenic endothelial cells, and bone marrow mesenchymal stem cells. Nestin filaments require vimentin and desmin for assembly into heterodimers and polymers. Upon differentiation, Nestin-containing filaments are replaced by cell type specific intermediate filament proteins, such as GFAP. Applications Reported: This Rat-401 (Rat401 (4D4)) antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This Rat-401 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells and by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval. This can be used at less than or equal to 10 μg/mL.

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

Specifications

• Antigen: Nestin
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P21263, Q6P5H2
• Gene Symbols: Nes
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18008, 25491
• Content And Storage: 4°C
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: Rat-401 (Rat401 (4D4))
• Conjugate: Unconjugated
• Gene: Nes
• Gene Alias: AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.