Neurogranin, Rabbit anti-Rat, Human, Polyclonal, Millipore Sigma™

Catalog No.	Quantity
07425I100UG	100 μg
07425I25UG	25 μg

Catalog No. 07425I100UG Supplier MilliporeSigma Supplier No. 07425I100UG

Description

Rabbit Polyclonal Antibody

Anti-Neurogranin Antibody, Cat. No. 07-425-I, is a highly specific rabbit polyclonal antibody that targets Neurogranin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Neurogranin (UniProt: Q92686; also known as Ng, RC3) is encoded by the NRGN gene (Gene ID: 4900) in human. Neurogranin is abundantly expressed in cerebral cortex, hippocampus, amygdala, and striatum. In neurons, it is found concentrated at dendritic spines. It acts as a third messenger and serves as a substrate for protein kinase C-mediated molecular cascades during synaptic development and remodeling. It contains an IQ domain (aa 26-347) and a collagen-like domain (aa 48-78). The IQ domain mediates its interaction with calmodulin and phosphatidic acid (PA). Neurogranin is intrinsically unstructured, however, upon binding with calmodulin, the IQ domain adopts a helical conformation. Neurogranin is phosphorylated at serine 36 by protein kinase C and following phosphorylation neurogranin is unable to bind either calmodulin or PA, which can lead to decline in memory-associated functions. Higher levels of neurogranin have also been reported in brains of elderly and is considered a factor in memory decline, independent of Alzheimer s disease. (Ref.: Javier Dı´ez-Guerra, F. (2010). Life. 62(8): 597-606; Casaletto, KB et al. (2017). Neurology 89(17); 1782-1788).

Specifications

• Antigen: Neurogranin
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Ng;RC3
• Gene Symbols: NRGN
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.
• Molecular Weight of Antigen: ∽15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Purification Method: Affinity Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001119653
• Test Specificity: This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.
• Target Species: Rat, Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG