Neuropilin-1 (CD304) Mouse anti-Human, Brilliant Violet 510, Clone: U21-1283, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 743129

Catalog No. BDB743129

Description

The U21-1283 monoclonal antibody specifically recognizes Neuropilin-1 (NRP1), also known as CD304, Blood dendritic cell antigen 4 (BDCA4), and Vascular endothelial cell growth factor 165 receptor (VEGF165R). CD304 is a type I transmembrane glycoprotein involved in the development of the nervous and cardiovascular systems. It mediates the interaction, growth, survival, and migration of a variety of normal and tumor cells. CD304 is expressed on neurons, thymocytes, regulatory T cells, a subset of T follicular helper cells, dendritic cells, endothelial cells, and certain tumor cells. Neuropilin-1 has a very short cytoplasmic domain and interacts with various coreceptors to form ligand-binding, signal-transducing receptor complexes. CD304 complexes with Plexin-A family members to bind chemorepellent Class 3 Semaphorins and guide neuronal axon growth. It also functions as a coreceptor with VEGFR2/CD309 to stimulate angiogenesis in response to VEGF165. CD304 mediates the interactions between some T cells and dendritic cells.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.

Specifications

• Antigen: Neuropilin-1 (CD304)
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Neuropilin, NRP, NP1, Blood dendritic cell antigen 4, BDCA-4, VEGF165R
• Immunogen: Recombinant Human Neuropilin-1
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human

• Clone: U21-1283
• Conjugate: Brilliant Violet 510
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse IgG_2b, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal