NF-H Antibody (NE14), Alexa Fluor™ 594, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP247834AF594

Catalog No. NP247834A94

Description

NF-H Monoclonal specifically detects NF-H in Human, Mouse, Rat, Porcine, Bovine, Chicken, Feline, Guinea Pig, Gerbil, Rabbit samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: NF-H
• Classification: Monoclonal
• Conjugate: Alexa Fluor 594
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: NEFH
• Immunogen: Crude neurofilament preparation from porcine spinal cord
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
• Content And Storage: Store at 4°C in the dark.

• Applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Clone: NE14
• Dilution: Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
• Gene Alias: 200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H
• Host Species: Mouse
• Quantity: 0.1 mL
• Gene ID (Entrez): 4744
• Target Species: Human, Mouse, Rat, Pig, Chicken, Feline, Guinea Pig, Gerbil, Primate, Rabbit
• Isotype: IgG1 κ