NFkB p65 Recombinant Rabbit Monoclonal Antibody (4-2H22L23), Invitrogen™

Rabbit Recombinant Monoclonal Antibody

Supplier:  Thermo Scientific 701079

Catalog No. PI701079

Description

Intact IgG appears on a non-reducing gel as ∽150 kDa band and upon reduction generating a ∽25 kDa light chain band and a ∽50 kDa heavy chain. Recombit rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombit rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

NF kappaB p65 belongs to the Rel family of transcription factors. NF kappaB homo and heterodimers are retained in an inert, non-DNA-binding form in the cytosol by inhibitory molecules in most cells. Activation of NF kappaB occurs predomitly through the phosphorylation and degradation of I kappaB proteins. The free NF kappaB translocates to the nucleus, where it upregulates expression from genes involved in developmental processes, cellular growth, apoptosis, and immune and inflammatory responses. In humans, the gene is located on the q arm of chromosome 11.

Specifications

• Antigen: NFkB p65
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: Q04206
• Gene Symbols: RELA
• Immunogen: Recombit protein corresponding to amino acids 375–551 of NF kappaB p65
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: ChIP assay, Immunohistochemistry (Paraffin), Western Blot
• Clone: 4-2H22L23
• Conjugate: Unconjugated
• Gene: RELA
• Gene Alias: nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)); v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; nf?b
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 5970
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid