Invitrogen™ NFYA Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA528990

Catalog No. PIPA528990

Description

Recommended positive controls: A431, HeLa, HepG2, NIH-3T3, JC, BCL-1, PC-12, Rat2. Predicted reactivity: Mouse (99%), Rat (98%), Pig (97%), Rhesus Monkey (91%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The NFYA gene encodes for one of the subunits of the Nuclear Factor Y (NF-Y) transcription factor, which plays a critical role in the regulation of various genes, particularly those containing the CCAAT box within their promoter regions. Structurally, NF-Y consists of a heterotrimeric complex made up of NF-YA, NF-YB, and NF-YC subunits. NF-YA serves as the sequence-specific DNA-binding component, whereas the NF-YB/NF-YC heterodimer forms a Histone Fold Domain crucial for DNA binding and stabilization. Functionally, NF-YA is pivotal in cell cycle regulation and neuronal development, influencing neural progenitor maintenance during brain morphogenesis. It has been implicated in various physiological processes, including response to plant stress and cancer progression. Specifically, NFYA gene activity is prevalent in proliferating cells and is often dysregulated in cancerous tissues, which highlights its potential as a therapeutic target. NFYA's expression in plant systems, such as in the Arabidopsis species, is modulated by environmental stresses like drought, indicating its broader impact on biological stress responses.

Specifications

• Antigen: NFYA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NFYA
• Gene Alias: AA407810; CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; CAAT-box DNA-binding protein subunit A; CBF-A; Cbf-b; CCAAT-binding transcription factor subunit A; CCAAT-binding transcription factor subunit B; FLJ11236; HAP2; HAP2 CCAAT-binding protein; nf-y; NFYA; NF-YA; Nuclear transcription factor Y subunit A; nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; nuclear transcription factor-Y alpha; SEZ10; SEZ-10; Transcription factor NF-Y, A subunit
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18044, 29508, 4800
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.19 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P18576, P23511, P23708
• Gene Symbols: NFYA
• Immunogen: Full length human NFYA Recombinant protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov