Invitrogen™ NFYB Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA531913

Catalog No. PIPA531913

Description

Recommended positive controls: HeLa, HeLa nuclear extract, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (92%), Pig (99%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein, histone-fold handshake motif in a manner analogous to the histone proteins H2B and H2A, respectively.

Specifications

• Antigen: NFYB
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NFYB
• Gene Alias: AA985999; CAAT box DNA-binding protein subunit B; CAAT-box DNA-binding protein subunit B; CBF-A; CBFB; CBF-B; CCAAT binding transcription factor of CBF-B/NFY-B; CCAAT-binding transcription factor subunit A; CCAAT-binding transcription factor subunit B; HAP3; nf-y; Nfya; Nfyb; NF-YB; nuclear transcription factor - Y beta; nuclear transcription factor Y subunit B; Nuclear transcription factor Y subunit beta; nuclear transcription factor Y, beta; nuclear transcription factor-Y beta; Transcription factor NF-Y, B subunit
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18045, 25336, 4801
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ChIP Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.42 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P25208, P63139, P63140
• Gene Symbols: NFYB
• Immunogen: Recombinant fragment corresponding to a region within amino acids 33 and 207 of Human NFYB.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG