NGAL Monoclonal Antibody (5G5), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific HYB2110102

Catalog No. HYB2110102

Description

HYB 211-01-02 is specific for human NGAL, native and denatured forms. No cross-reaction to NGAL from other species has been detected.  HYB 211-01-02 has been successfully used in Western blotting, ELISA and Immunohistochemistry procedures. HYB 211-01-02 reacts strongly with NGAL. Strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against the antigen. In immunohistochemical staining of paraformaldehyde fixed neutrophils HYB 211-01-02 recognizes NGAL. In western blotting HYB 211-01-02 reacts with NGAL in both reduced as well as unreduced form. The HYB 211-01-02 immunogen is recombit human NGAL in monomer and dimer forms absorbed to aluminum hydroxide gel. The epitope specificity of HYB 211-01-02 differs from that of HYB 211-05-02, and differs from HYB 211-02-02 but overlaps.

NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.

Specifications

• Antigen: NGAL
• Classification: Monoclonal
• Concentration: 0.94 mg/mL
• Gene: LCN2
• Gene Alias: Neutrophil gelatinase-associated lipocalin, p25, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 3934
• Content And Storage: 4° C, store in dark
• Form: Liquid

• Applications: ELISA, Immunohistochemistry, Western Blot
• Clone: 5G5
• Conjugate: Unconjugated
• Gene Accession No.: P80188
• Gene Symbols: LCN2
• Immunogen: Recombit human NGAL in monomer and dimer forms
• Quantity: 200 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ