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Cytiva NHS-Activated Sepharose™ 4 Fast Flow Affinity Media

Use for coupling amino-containing smaller proteins and peptides.  Cytiva Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH.

Supplier:  Cytiva 17090601

Catalog No. 45-002-965

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  • Composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides
  • NHS (N-hydroxysuccinimide) coupling forms chemically stable amide bond with ligands containing primary amino groups
  • Resin provides spacer arm to coupled ligand
  • Fast Flow matrix gives high productivity
  • Coupled product is very stable, especially at high pH
  • Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development



25 mL
Stable in commonly used aqueous Buffers provided that the ligand withstand the pH or the chemical environment
NHS-Activated Sepharose 4 Fast Flow Media
45 to 165 μm
25 cm
4% Cross-Linked Agarose
100% Isopropanol
For Coupling Amino-Containing Smaller Proteins and Peptides
2°C to 8°C
150 to 250 cm/Hoμr
9.84 in.
100 kPa
3 to 13
N-hydroxysuccinimide (NHS) Activated




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