Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Catalog Number	Mfr. No.	Description	Quantity
PI46409	Thermo Scientific™ 46409	Pierce NHS-Fluorescein	1 g
PI46410	Thermo Scientific™ 46410	Pierce NHS-Fluorescein	100 mg

Description

Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

• Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery

Specifications

• Description: Pierce NHS-Fluorescein
• Solubility: DMF (Dimethylformamide)
• Chemical Reactivity: Amine
• Label or Dye: FITC (Fluorescein)
• Product Line: Pierce™
• Excitation: 494

• Product Type: NHS-Fluorescein
• Content And Storage: Store desiccated at -20°C, protect from moisture
• Reactive Moiety: Active Ester, Succinimidyl Ester, NHS Ester
• Label Type: Classic Dyes
• Emission: 518

For Research Use Only. Not for use in diagnostic procedures.