Nicotinamide N-methyltransferase, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Catalog No.	Quantity
ABE178825UG	25 μg

Catalog No. ABE178825UG Supplier MilliporeSigma Supplier No. ABE178825UG

Description

Rabbit Polyclonal Antibody

Anti-Nicotinamide N-methyltransferase Antibody, Cat. No. ABE1788, is a highly specific rabbit polyclonal antibody that targets human Nicotinamide N-methyltransferase and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Nicotinamide N-methyltransferase (UniProt: P40261; also known as EC: 2.1.1.1) is encoded by the NNMT gene (Gene ID: 4837) in human. Nicotinamide N-methyltransferase is a monomeric, cytoplasmic enzyme that belong to the class I -like SAM-binding methyltransferase superfamily. It catalyzes the N-methylation of nicotinamide and other pyridines with S-Adenosyl-L-methionine (SAM)) serving as the methyl donor. It has two SAM binding sites (aa 63-64 and 142-143). Its catalytic activity is important for biotransformation of many drugs and xenobiotic compounds. Nicotinamide N-methyltransferase is predominantly expressed in the liver. A lower level of expression is seen in the kidney, lung, skeletal muscle, placenta, and heart and it is not detected in the brain or pancreas. Nicotinamide N-methyltransferase inhibition is shown to increases adipose SAM and NAD(+) levels and upregulate ornithine decarboxylase and spermidine-spermine N(1)-acetyltransferase activity and expression. (Ref.: Kraus, D et al. (2014). Nature. 508 (7495); 258-62).

Specifications

• Antigen: Nicotinamide N-methyltransferase
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: 1μg/mL from a representative lot detected Nicotinamide N-methyltransferase in 10μg of  human kidney lysate and HEK293 cell lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nicotinamide N-methyltransferase in human liver, human kidney, and human pancreas tissues.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: EC 2.1.1.1
• Gene Symbols: NNMT
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Nicotinamide N-methyltransferase.
• Molecular Weight of Antigen: ∽30 kDa observed; 29.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Purification Method: Affinity Purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody detects human Nicotinamide N-methyltransferase. It targets an epitope within 15 amino acids from the internal region.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG