Invitrogen™ Ninein Polyclonal Antibody

Catalog No.	Quantity
PIPA584846	100 μL

Catalog No. PIPA584846 Supplier Invitrogen™ Supplier No. PA584846

Description

Rabbit Polyclonal Antibody

Immunogen sequence: EPGLVMSSCL DEPATEFFGN TAEQTEQFLQ QNRTKQVEGV TRRHVLSDLE DDEVRDLGST GTSSVQRQEV KIEESEASVE GFSELENSEE TRTESWELKN Highest antigen sequence indentity to the following orthologs: Mouse - 59%, Rat - 56%.

Ninein is a component of subdistal appendages of the mother centriole, localized specifically in the pericentriolar matrix of the centrosome. It may be involved in microtubule minus-end capping, centriole positioning, and protein anchoring. Recent studies have shown that the microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function. At least five human ninein isoforms with divergent C-terminal domains and two mouse isoforms have been reported. Data indicates that the combined action of domains (C-terminal and N-terminal) might, in the absence of coiled coil region, be sufficient to localize ninein to the mother centriole. Microinjection of antibodies against ninein into metaphase HeLa cells can disrupt the reformation of tubular conformation of proteins within the centrosome following cell division, and consequently lead to dispersal of centrosomal material throughout the cytosol. MTOC (microtubule organizing center) function can be disrupted when anti-ninein antibodies are injected into postmitotic cells. Antibodies against ninein are present in sera from patients with autoimmune diseases that develop autoantibodies to centrosomal proteins.

Specifications

• Antigen: Ninein
• Applications: Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.3 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene: NIN
• Gene Accession No.: Q8N4C6
• Gene Alias: 3110068G20Rik; AI385615; AU024711; glycogen synthase kinase 3 beta-interacting protein; GSK3B-interacting protein; hNinein; KIAA1565; mKIAA1565; Nin; Ninein; ninein (GSK3B interacting protein); ninein centrosomal protein; SCKL7
• Gene Symbols: NIN
• Host Species: Rabbit
• Immunogen: Recombinant protein corresponding to Human NIN. Recombinant protein control fragment (Product #RP-107764).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 51199
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG