MilliporeSigma™ Ninein, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABN1720

Catalog No. ABN1720MI

Description

Ninein (UniProt Q61043; also known as Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein) is encoded by the Nin (also known as Kiaa1565) gene (Gene ID 18080) in murine species. Ninein is a microtubule (MT) minus-end-binding protein that helps anchor the MTs to the centrosome. Ninein is also found in noncentrosomal locations in association with the minus-end of noncentrosomal MTs present in polarized cells. Ninein is essential for the self-renewal and maintenance of apical progenitors (APs) in developing mouse neocortex by anchoring of MTs to the centrosome, while only noncentrosomal ninein is seen in cortical neurons where it is localized to the axons and the somatodendritic compartment. Centrosome-associated ninein plays an important role in stabilizing the radial MT asters, while noncentrosomal ninein plays an MT-stabilizing role in the axons of neurons. Ninein is a downstream target of the transcription factor Sip1 involved in CNS development. Re-expression of ninein in Sip1-deficient neurons or overexpressing ninein in wild-type neurons significantly increases their resistance to MT depolymerization.

Specifications

• Antigen: Ninein
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Nin, Kiaa1565
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This polyclonal antiserum targets region between EF-hands 2&5 present in all murine and human ninein spliced isoforms reported by UniProt (Q61043 & Q8N4C6).
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
• Gene Accession No.: Q61043
• Immunogen: His-tagged recombinant mouse ninein fragment corresponding to the region between EF-hands 2&5.
• Quantity: 100 μL
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_001273008
• Target Species: Human
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.