MilliporeSigma™ NKCC1/2, Mouse, Unlabeled, Clone: T4,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1237

Catalog No. MABS1237MI

Description

Solute carrier family 12 member 1 (UniProt Q13621; a.k.a. Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2) and solute carrier family 12 member 2 (UniProt P55011; a.k.a. Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1) are encoded by the SLC12A1 (a.k.a. NKCC2; Gene ID 6557) and SLC12A2 (a.k.a. NKCC1; Gene ID 6558) gene, respectively, in human. NKCC1 (SLC12A2) and NKCC2 (SLC12A1) belong to the subfamily of cation-chloride cotransporters (CCCs) that mediate electroneutral transport of sodium, potassium and chloride across the plasma membrane and are inhibited by bumetanide and furosemide-type of compounds. NKCCs play an important role in cell volume regulation, where NKCC2 is involved in reabsorption of salt and osmotically-obliged water in renal epithelial cells, while NKCC1 has a major impact on intracellular chloride ion concentration ([Cl-]i) in vascular smooth muscle cells (VSMCs) and neuronal cells. In primary hypertension, NKCC1 activity is increased in VSMCs and paraventricular nucleus (PVN) neurons, leading to elevated peripheral resistance in systemic circulation and activation of sympathetic nerve system (SNS), respectively. Human NKCC1 and NKCC2 are 12-transmembrane proteins having their N- and C-terminal ends exposed at the cytoplasmic side (a.a. 1-285 and 921-1212 of NKCC1; a.a. 1-177 and 814-1099 of NKCC2).

Specifications

• Antigen: NKCC1/2
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: P55011
• Immunogen: Recombinant human NKCC1 C-terminal fragment.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_001037.1
• Target Species: Avian
• Form: Purified

• Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
• Clone: T4
• Conjugate: Unconjugated
• Gene: SLC12A1, SLC12A2, NKCC2, NKCC1
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone T4 recognizes both NKCC1 and NKCC2 by targeting a C-terminal region epitope exposed only under denaturing condition, and unaccessible for antibody binding when NKCC assumes its native conformation (Lytle, C., et al. (1995). Am. J. Physiol. 269(6 Pt 1):C1469-C1505).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.