NKp46 (CD335), Mouse anti-Human, Clone: D2-9A5, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABF2058100UG

Catalog No. MABF2058100

Description

Anti-NKp46 (CD335), clone D2-9A5 , Cat. No. MABF2058, is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 1 (NKp46) and has been tested for use in and ELISA, and Flow Cytometry.

Natural cytotoxicity triggering receptor 1 (UniProt: O76036; also known as Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, hNKp46, CD335) is encoded by the NCR1 (also known as LY94) gene (Gene ID: 9437) in human. NK-p46 is a single-pass type I membrane protein that belongs to the natural cytotoxicity receptor (NCR) family and serves as a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis and elimination of virus-infected cells. It is considered as a primary activating receptor mediating NK cell antiviral function. It is involved in the functional recognition of viral HA and hemagglutinin-neuraminidases. NKp46 is shown to be predominantly expressed in the nasal lavage of patients infected with respiratory viruses, but not in normal healthy subjects. Following the initiation of an NK-target cell interaction, NKp46 is reported to cluster at the cell membrane, specifically at the immune synapse where it mediates cytoskeletal rearrangement and cellular polarization. Human NK-p46 is synthesized with a signal peptide of 21 amino acids, which is subsequently cleaved off. The mature form has an extracellular domain (aa 22-258), a helical domain (aa 259-279), and a cytoplasmic domain (aa 280-304). It also has two Ig-like domains (aa 34-118 and 129-211) termed as D1 and D2. Five different isoforms of NKp46 have been described that are produced by alternative splicing. (Ref.: Shemer-Avni, Y., et al., (2017). Front. Immunol. 8 (Article 161); 1-10).

Specifications

• Antigen: NKp46 (CD335)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: NCR1;LY94
• Immunogen: Human IgG1-Fc - fused to a recombinant fragment corresponding to 135 amino acids from the ectodomain region of human NKp46.
• Quantity: 100 μg
• Research Discipline: Inflammation & Immunology
• Gene ID (Entrez): NP_001138929
• Target Species: Human
• Form: Purified

• Applications: ELISA, Flow Cytometry
• Clone: D2-9A5
• Dilution: Flow Cytometry Analysis: A representative lot detected NKp46 (CD335) in Flow Cytometry applications (Shemer-Avni, Y., et. al. (2017). Front Immunol. 8:161).Flow Cytometry Analysis: 4μg/mL from a representative lot detected NKp46 (CD335) in NK92 cells transfected with human NKp46 (Courtesy of Angel Porgador, Ph.D., Faculty of Health Sciences, Ben Gurion university of the Negev, Israel).ELISA Analysis: A representative lot detected NKp46 (CD335) in ELISA applications (Shemer-Avni, Y., et. al. (2017). Front Immunol. 8:161).
• Gene Alias: Natural cytotoxicity triggering receptor 1;Lymphocyte antigen 94 homolog;NK cell-activating receptor;Natural killer cell p46-related protein;NK-p46;hNKp46;CD335
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone D2-9A5 recognizes the whole NKp46 and NKp46-D2, but does not recognize NKp46-D1. It targets an epitope with in 135 amino acids from the extracellular domain.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG1 κ