Invitrogen™ Nkx2.6 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA575416

Catalog No. PIPA575416

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Members of the NK-2 family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas.Nkx-2.5, also designated cardiac specific homeobox protein (Csx), is a homolog of the Drosophila tinman protein and is essential for normal cardiovascular development. Expression of Nkx-2.5 during cardiomyogenesis is required for cardiac septation, in which a single atrium and ventricle are separated into four chambers. Nkx-2.5 binds to DNA as a monomer, a homodimer or as a heterodimer with Nkx-2.3 or Nkx-2.6, which suggests that the specific protein-protein interactions of Nkx-2.5 are involved in its transcriptional regulatory function. Nkx-2.6, also a homolog of the Drosophila tinman protein, is expressed in the caudal pharyngeal pouches,the caudal heart progenitors, the sinus venosus, the outflow tract of the heart and in a short segment of the gut between stages E8.5 and E10.5 of embryogenesis. Expression of Nkx-2.6 overlaps with that of Nkx-2.5 in the pharynx and heart. However, Nkx-2.6 mutant mice are viable and fertile, which suggests that Nkx-2.6 plays a compensatory function to Nkx-2.5.

Specifications

• Antigen: Nkx2.6
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NKX2-6
• Gene Alias: AV044928; CSX2; CTHM; Drosophila NK2 transcription factor related, locus 6; homeobox protein NK2 homolog F; homeobox protein NK-2 homolog F; homeobox protein NKX2.6; homeobox protein Nkx-2.6; NK2 homeobox 6; NK2 transcription factor related, locus 6; Nkx2.6; Nkx-2.6; Nkx2-6; Nkx2f; NKX4-2; tinman; tinman paralog; Tix
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 137814, 18092, 364418
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide; pH 7.2
• Gene Accession No.: A6NCS4, P43688
• Gene Symbols: NKX2-6
• Immunogen: Synthetic peptide corresponding to amino acids 111-160 of Human Nkx2.6.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG