Invitrogen™ NLK Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521877

Catalog No. PIPA521877

Description

Recommended positive controls: NLK-transfected 293T, A431, H1299, HeLa, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (98%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

NLK is a serine/threonine kinase involved in signal transduction and cellular communication. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors.

Specifications

• Antigen: NLK
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NLK
• Gene Alias: AI194375; LAK1; nemo like kinase; nemo-like kinase; NLK; Protein LAK1; RGD1561602; serine/threonine protein kinase NLK; serine/threonine-protein kinase NLK
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18099, 51701
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O54949, Q9UBE8
• Gene Symbols: NLK
• Immunogen: Recombinant fragment corresponding to a region within amino acids 234 and 527 of NLK (Uniprot ID#Q9UBE8).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov