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Invitrogen™ nNOS Monoclonal Antibody (J23), FITC
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Catalog No. PIMA545958
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100 μg
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Catalog No. Quantity
PIMA545958 100 μg
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Catalog No. PIMA545958 Supplier Invitrogen™ Supplier No. MA545958
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Description

Mouse Monoclonal Antibody

A 1:1,000 dilution of MA5-45958 was sufficient for detection of NOS1/Nnos in 7.5 μg of Mouse Brain Cerebellum by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 160.97 kDa.

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
TRUSTED_SUSTAINABILITY

Specifications

Antigen nNOS
Applications ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry
Classification Monoclonal
Clone J23
Concentration 1 mg/mL
Conjugate FITC
Formulation PBS, 9.1mM sodium bicarbonate with 640.91mM DMSO, 136.36mM ethanolamine and no preservative; pH 7.4
Gene NOS1
Gene Accession No. P29475, P29476, Q9Z0J4
Gene Alias 2310005C01Rik; bNOS; bNOS1; Brain NOS; Constitutive NOS; IHPS1; NC-NOS; neuronal nitric oxide synthase; neuronal nitric oxide synthase NOS1; neuronal NOS; nitric oxidase synthase; nitric oxide synthase; nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; N-NOS; nNOS; NOS type I; NO; NOS; NOS Type 1; NOS type I; Nos1; Nos-1; NOS-I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
Gene Symbols NOS1
Host Species Mouse
Immunogen Partial Human Recombinant NOS1 Protein.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 18125, 24598, 4842
Target Species Human, Mouse, Rat
Content And Storage 4°C, store in dark
Product Type Antibody
Form Liquid
Isotype IgG2a
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Safety and Handling

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