NNT1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P186BT25	Invitrogen™ 500P186BT25UG	25 μg
500P186BT50	Invitrogen™ 500P186BT50UG	50 μg
50-271-8627	Invitrogen™ 500P186BT500UG	500 μg

Description

AA Sequence of recombinant protein: MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NNT-1/BCSF-3. Anti-Human NNT-1/BCSF-3-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NNT-1/BCSF-3 (500-P186) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hNNT-1/BCSF-3. Western Blot: To detect Human NNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NNT-1/BCSF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P186BT-1MG will be provided as 2 x 500 μg

NNT-1/BCSF-3 is a neurotrophic factor with B-cell-stimulating capabilities. Expressed in the lymph nodes and spleen, NNT-1/BCSF-3 activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member b (LIFR-b) by binding and inducing the tyrosine phosphorylation of these receptors. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss, and B cell hyperplasia associated with increases in serum IgG and IgM.

Specifications

• Antigen: NNT1
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q9UBD9
• Gene ID (Entrez): 23529
• Immunogen: E.coli-derived, 22.4 kDa Recombinant Human NNT-1/BCSF-3.
• Purification Method: Antigen Affinity Chromatography
• Gene: CLCF1

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: b cell-stimulating factor 3; B-cell stimulating factor 3; B-cell stimulatory factor 3; B-cell-stimulating factor 3; Bsf3; BSF-3; Cardiotrophin-like cytokine factor 1; CISS2; Clc; CLCF1; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT1; NNT-1; NNT-1/BSF-3; Novel neurotrophin-1; NR6
• Gene Symbols: CLCF1
• Primary or Secondary: Primary
• Content And Storage: -20°C