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PeproTech NNT1 Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P1861MG
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50 μg
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500P1861MG 1 mg
500P18650UG 50 μg
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Catalog No. 500P1861MG Supplier PeproTech Supplier No. 500P1861MG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NNT-1/BCSF-3. Anti-Human NNT-1/BCSF-3-specific antibody was purified by affinity chromatography employing an immobilized Human NNT-1/BCSF-3 matrix. Sandwich ELISA: To detect Human NNT-1/BCSF-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NNT-1/BCSF-3 (500-P186Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human NNT-1/BCSF-3. Western Blot: To detect Human NNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NNT-1/BCSF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions

NNT-1/BCSF-3 is a neurotrophic factor with B-cell-stimulating capabilities. Expressed in the lymph nodes and spleen, NNT-1/BCSF-3 activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member b (LIFR-b) by binding and inducing the tyrosine phosphorylation of these receptors. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss, and B cell hyperplasia associated with increases in serum IgG and IgM.
TRUSTED_SUSTAINABILITY

Specifications

Antigen NNT1
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene CLCF1
Gene Accession No. Q9UBD9
Gene Alias b cell-stimulating factor 3; B-cell stimulating factor 3; B-cell stimulatory factor 3; B-cell-stimulating factor 3; Bsf3; BSF-3; Cardiotrophin-like cytokine factor 1; CISS2; Clc; CLCF1; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT1; NNT-1; NNT-1/BSF-3; Novel neurotrophin-1; NR6
Gene Symbols CLCF1
Host Species Rabbit
Immunogen E.coli-derived Recombinant Human NNT-1/BCSF-3.
Purification Method Antigen affinity chromatography
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 23529
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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