Invitrogen™ Nonspecific Cytotoxic Cell Monoclonal Antibody (5C.6)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1760

Catalog No. MA1760

Description

MA1-760 detects nonspecific cytotoxic cells (NCC) from catfish and tilapia tissues and also detects natural killer/lymphokine activated killer (NK/LAK) cells from rat, bovine, equine, canine and porcine tissues. MA1-760 has been successfully used in Western blot, cytotoxic inhibition studies with NCC/NK/LAK cells, FACS, immunoprecipitation, panning, and ELISA procedures. By Western blot, this antibody detects a 32 kDa protein representing nonspecific cytotoxic cell receptor protein. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation. The MA1-760 immunogen is purified NCC cells from catfish.

Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.

Specifications

• Antigen: Nonspecific Cytotoxic Cell
• Classification: Monoclonal
• Concentration: 0.73 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Target Species: Multi-species
• Product Type: Antibody
• Isotype: IgM

• Applications: ELISA, Flow Cytometry, Inhibition Assays, Immunoprecipitation, Western Blot
• Clone: 5C.6
• Conjugate: Unconjugated
• Gene Alias: Natural Killer Cell; NCC/NK
• Immunogen: Purified NCC cells from catfish.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid