Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit

Description

Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.

Thermo Scientific™ North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

• Sensitivity equal to or greater than ³²P
• Amenable to many formats
• High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
• Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user

Specifications

• Content And Storage: Sufficient For: 1000 cm² of membrane (10 mini-gel blots)
  • Stabilized Streptavidin-HRP, 1.5 mL
  • Luminol/Enhancer Solution, 80 mL
  • Stable Peroxide Solution, 80 mL
  • Hybridization Buffer, 125 mL
  • Hybridization Stringency Wash Buffer (2X), 375 mL
  • Blocking Buffer, 500 mL
  • Wash Buffer (4X), 500 mL
  • Substrate Equilibration Buffer, 500 mL
  
  Upon receipt, store kit at 4°C.
• Detection Method: Chromogenic
• Format: Kit
• Product Line: North2South™
• Product Type: Hybridization and Detection Kit
• Quantity: 2160 mL Kit