Invitrogen™ NOSIP Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA530613

Catalog No. PIPA530613

Description

Recommended positive controls: 293T, A431, Raji, mouse brain. Predicted reactivity: Mouse (89%), Rat (90%), Rhesus Monkey (99%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1. 2, Nav1. 3, Nav1. 5, Nav1. 7, Nav1. 8, Kv1. 3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.

Specifications

• Antigen: NOSIP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NOSIP
• Gene Alias: 2310061K06Rik; CGI-25; E3 ubiquitin-protein ligase NOSIP; eNOS-interacting protein; nitric oxide synthase interacting protein; nitric oxide synthase-interacting protein; Nosip; RING-type E3 ubiquitin transferase NOSIP
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 51070, 66394
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.53 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9D6T0, Q9Y314
• Gene Symbols: NOSIP
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 300 of Human NOSIP.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov